High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells
نویسندگان
چکیده
DNA site-specific recombinases (SSRs) such as Cre, FLPe, and phiC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has only been observed with Cre recombinase. Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and PhiC31 (PhiC31o) SSRs, which result in recombination efficiencies similar to Cre.
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عنوان ژورنال:
- PLoS ONE
دوره 2 شماره
صفحات -
تاریخ انتشار 2007